Journal: Cells

Article Title: IL-3-Induced Immediate Expression of c- fos and c- jun Is Modulated by the IKK2-JNK Axis

doi: 10.3390/cells11091451

Figure Lengend Snippet: Effects of IKK1 and/or IKK2 KO on IL-3-induced expression of IEGs. ( A ) IKK1 and IKK2 protein levels in Ba/F3 parental cells (WT) and CRISPR/Cas9-mediated IKK1 KO, IKK2 KO, and IKK1/2 DKO cells. Whole-cell lysates were immunoblotted with the indicated Abs. A dagger denotes the detection of IKK2 by potential cross-reactivity of the anti-IKK1 Ab. ( B – D ) Relative levels of expression of c- fos ( B ), c- jun ( C ), and c- myc ( D ) mRNAs measured 20, 40, and 40 min, respectively, after IL-3 stimulation of Ba/F3 parental, IKK1 KO, IKK2 KO, and IKK1/2 DKO cells. * p < 0.05; n.s., not significant, as assessed by the Kruskal-Wallis test with the Steel-Dwass test. Each dot represents the result from independent experiments ( n = 5). Means are indicated by bars.

Article Snippet: To generate IKK1 or IKK2 single KO clones, 5 × 10 6 parental Ba/F3 cells were electroporated with 120 μg of a Cas9 expression plasmid (41815, Addgene), 120 μg of a sgRNA expression plasmid targeting IKK1 or IKK2 , and 1 μg of a plasmid encoding a puromycin resistant gene using the Gene Pulser Xcell (1652660J1, Bio-Rad, Richmond, CA, USA).

Techniques: Expressing, CRISPR

Journal: Neuron

Article Title: Visual intracortical and transthalamic pathways carry distinct information to cortical areas

doi: 10.1016/j.neuron.2021.04.017

Figure Lengend Snippet:

Article Snippet: For the experiments involving optogenetic manipulations ( , , and ), AAV1.Syn.DIO.ChrimsonR.tdTomato (120 nl, 3.9 × 10 12 vg/mL, 1:5 dilution in saline solution, UNC vector core, Addgene plasmid # 62723, from Edward Boyden) or AAV1.CAG.DIO.tdTomato (control, 120 nl, 2.6 × 10 13 vg/mL, diluted 1:5 in saline, Addgene viral prep # 28306-AAV1, from Edward Boyden) was injected into AL, PM (single injection) or V1 (5 to 7 injections).

Techniques: Plasmid Preparation, Recombinant, Software